Mini Rapid Plasmid DNA Preparation

Application: High and Low Copy Plasmid DNA Purification

Target: plasmid DNA

CE certified: No, for research use only

Technology: Silica membrane technology

Brand: NucleoSpin

Format: Miniprep

Packaging unit: 10 slides, 50 slides, 250 slides

Handling: Centrifugation, vacuum

Clarification of lysates: Centrifugation

Automated use: No

Sample material: Bacteria, E. coli

Sample quantity: 1 to 5 ml (high copy), 6 to 10 ml (low copy)

Vector size: <25 kbp

Typical yield: 25-45 µg

Theoretical binding capacity: 60 µg

Typical A260 / A280 Purity: 1.8−1.85

Endotoxin level: > 50 EU / µg DNA

Elution volume: 50 µL

Preparation time: 20 min / 6 preparations

Typical downstream application: Cloning, PCR, restriction analysis, sequencing, cell transfection

Storage temperature: 15-25 ° C

Shelf life (from production): 27 months

Hazardous material: Yes

The Thermo Scientific GeneJET Plasmid Miniprep Kit uses a unique silica-based membrane technology in the form of a convenient spin column. The kit recovers up to 20 µg of high copy number plasmid DNA per isolation procedure.

Reflexes

• Efficient: high yields of up to 20 µg of high-quality plasmid DNA
• Quick: the procedure takes less than 14 minutes
• Convenient – no phenol/chloroform extraction or alcohol precipitation required
• Pure-purified DNA is ready to use right out of the box

Applications

• Rapid isolation of high purity plasmid DNA suitable for all conventional molecular biology procedures, including:
• FastDigest or conventional restriction digestion
• Automated fluorescent and radioactive sequencing
• PCR
• In vitro transcription
• Transformation

Beginning

A bacterial culture is collected and smooth. The lysate is then cleared by centrifugation and applied to the silica column to selectively bind DNA molecules at a high salt concentration. The adsorbed DNA is washed to remove contaminants and the pure plasmid DNA is eluted in a small volume of elution buffer or water. The purified DNA is ready for immediate use in all molecular biology procedures, such as conventional and rapid digestion with restriction enzymes, PCR, in vitro transcription, transformation, and automated sequencing. Components Resuspension solution Lysis solution Neutralization solution Wash solution (concentrated) RNase A solution Elution buffer Spin columns Collection tubes Detailed protocol